Microfiltration adopted by focus and diafiltration on ultrafiltration membranes (Biomax-100, Biomax-10 and Ultracel-10) was used to get well extracellular laccase (EC 1.10.3.2) from tradition broth of wood-rotting fungus Cerrena unicolor. Feed, permeate, retentate and membrane wash-out options have been analysed for the presence of laccase, proteases, protein and brown impurities.
A straightforward, low-cost and short-term process was proposed to acquire retentates with low yields of whole proteins (lower than 14%), proteases exercise (lower than 15%) and brown impurities (from 2% to 29%) with a simultaneous laccase restoration above 73%. The diploma of laccase purification assorted from 6.7 to 11.Zero and trusted the kind of membrane used and content material of brown pigments within the feed.
Subsequent protein precipitation with chilly acetone elevated the diploma of purification about twice and diminished proteases and brown impurities to some extent. Ultracel-10 membrane was really useful as the very best resolution to stop fouling of membranes and to acquire laccase-enriched fraction with a really low content material of proteases and brown pigments.
Mechanism concerned within the evolution of bodily irreversible fouling in microfiltration and ultrafiltration membranes used for consuming water remedy.
Management of membrane fouling is essential for extra environment friendly use of membranes in water remedy. Management of bodily irreversible fouling, which is outlined as fouling that requires chemical cleansing to be cancelled, is especially essential for discount of operation price in a membrane course of. On this research, a long-term filtration experiment utilizing three several types of MF and UF membranes was carried out at an present water purification plant, and the evolution of bodily irreversible fouling was investigated.
The experimental outcomes demonstrated that the extent of bodily irreversible fouling differed considerably relying on the membrane sort. Cleansing of the fouled membranes with numerous chemical reagents demonstrated that natural matter was primarily chargeable for bodily irreversible fouling. Natural matter that had precipitated bodily irreversible fouling within the long-term operation was desorbed from the fouled membranes and was subjected to Fourier remodel infrared and 13C nuclear magnetic resonance analyses. These analyses revealed that carbohydrates have been dominant within the membrane foulant no matter the kind of membrane.
Primarily based on measurements of molecular weight distribution of natural matter within the feedwater and the permeates from the membranes, a two-step fouling mechanism is proposed to clarify the dominance of carbohydrates within the foulant: hydrophobic (humic-like) parts with small molecular weight are first adsorbed on the membrane and, consequently, slender the scale of micro-pores of membranes, after which hydrophilic (carbohydrate-like) compounds with bigger molecular weight plug the narrowed pores or the hydrophilic compounds are adsorbed onto the membrane floor conditioned by the hydrophobic parts.
International mannequin for optimizing crossflow microfiltration and ultrafiltration processes: a brand new predictive and design device.
A world mannequin and algorithm that predicts the efficiency of crossflow MF and UF course of individually or together within the laminar move regime is offered and efficiently examined. The mannequin accounts for solute polydispersity, ionic atmosphere, electrostatics, membrane properties and working situations. Pc packages have been written in Fortran 77 for various variations of the mannequin algorithm that may optimize MF/UF processes quickly when it comes to yield, purity, selectivity, or processing time.

The mannequin is validated efficiently with three take a look at circumstances: separation of bovine serum albumin (BSA) from hemoglobin (Hb), seize of immunoglobulin (IgG) from transgenic goat milk by MF, and separation of BSA from IgG by UF. These comparisons reveal the potential of the worldwide mannequin to conduct life like in silico simulations of MF and UF processes.
This mannequin and algorithm ought to show to be a useful approach to quickly design new or optimize present MF and UF processes individually or together in each pressure-dependent and pressure-independent regimes.
Effluent remedy by multi-media filtration, microfiltration and ultrafiltration: outcomes of a pilot investigation at WWTP Hoek van Holland.
Upgrading of wastewater remedy plant (WWTP) effluent as part of the Dutch governmental coverage to shut the water cycle has rising curiosity now. The Water Board Hoogheemraadschap van Delfland along with the mission workforce of Witteveen + Bos Consulting Engineers, Delft College of Know-how and Rossmark water remedy investigated the reuse potentialities of WWTP effluent within the area of Delfland. Due to this fact pilot analysis was carried out at WWTP Hoek van Holland making use of totally different filtration methods: multi-media filtration, micro- and ultrafiltration.
The outcomes present secure course of performances of the totally different filtration methods when correct pre-treatment was utilized. For microfiltration the filtration traits have been strongly influenced by particles which weren’t retained within the multi-media filter. For ultrafiltration the filtration traits have been strongly influenced by natural parts < 0.2 microm.
Storage vial for electrodes |
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51343320 | Scientific Laboratory Supplies | EACH | EUR 8.78 |
Labels Vial Top 9.53mm |
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LAB2142 | Scientific Laboratory Supplies | PK300 | EUR 55.06 |
Nalgene 50 Vial Holder |
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CRY8326 | Scientific Laboratory Supplies | EACH | EUR 51.87 |
Vial picker for cryotubes |
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DD390784A | Scientific Laboratory Supplies | PK10 | EUR 4.9 |
Cryobox for biopsy vial |
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DD64052 | Scientific Laboratory Supplies | PK12 | EUR 156.18 |
Eppendorf CryoStorage Vial 0.5mL |
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E0030079400 | Scientific Laboratory Supplies | PK960 | EUR 1238.04 |
Eppendorf CryoStorage Vial 1.0mL |
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E0030079418 | Scientific Laboratory Supplies | PK960 | EUR 1279.08 |
Eppendorf CryoStorage Vial 1.5mL |
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Eppendorf CryoStorage Vial 2.0mL |
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Eppendorf CryoStorage Vial 4.0mL |
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E0030079442 | Scientific Laboratory Supplies | PK480 | EUR 759.24 |
10mL Vial Clear CrimpNeck |
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CHR2518 | Scientific Laboratory Supplies | PK1000 | EUR 308.94 |
10mL Headspace Vial Amber |
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CHR2520 | Scientific Laboratory Supplies | PK1000 | EUR 348.84 |
100mL CrimpNeck Vial Clear |
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CHR2524 | Scientific Laboratory Supplies | PK1056 | EUR 595.08 |
2mL CrimpNeck Vial Clear |
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CHR2532 | Scientific Laboratory Supplies | PK1000 | EUR 180.12 |
2mL CrimpNeck Vial Amber |
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CHR2534 | Scientific Laboratory Supplies | PK1000 | EUR 162.2 |
1.1mL CrimpNeck Vial Clear |
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0.9mL CrimpNeck Vial Clear |
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CHR2578 | Scientific Laboratory Supplies | PK1000 | EUR 598.5 |
20mL Headspace-Vial Clear |
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20mL Headspace-Vial Amber |
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20mL Headspace-Vial Clear |
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20mL Headspace-Vial Amber |
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CHR2586 | Scientific Laboratory Supplies | PK1000 | EUR 391.02 |
50mL CrimpNeck Vial Clear |
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CHR2598 | Scientific Laboratory Supplies | PK1000 | EUR 688.56 |
2mL ScrewNeck Vial Clear |
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2mL ScrewNeck Vial Amber |
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CHR2662 | Scientific Laboratory Supplies | PK1000 | EUR 177.84 |
4mL ScrewNeck Vial Clear |
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CHR2668 | Scientific Laboratory Supplies | PK1000 | EUR 172.14 |
20mL Precision Thread Vial |
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CHR2676 | Scientific Laboratory Supplies | PK1000 | EUR 418.38 |
Snap Top Vial 0.9mL |
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Insert WO Vial 0.1mLBottomSpring |
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CHR2976 | Scientific Laboratory Supplies | PK1000 | EUR 750.12 |
Insert WO Vial 0.4mL |
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CHR2988 | Scientific Laboratory Supplies | PK500 | EUR 87.42 |
Vial Injection Stopper 20mm |
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VIA1134 | Scientific Laboratory Supplies | PK1500 | EUR 289.56 |
Vial Seal 13mm Aluminium |
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VIA1136 | Scientific Laboratory Supplies | PK500 | EUR 83.22 |
Eppendorf Sterile CryoStorage Vial 0.5mL |
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E0030079450 | Scientific Laboratory Supplies | PK960 | EUR 1279.08 |
Eppendorf Sterile CryoStorage Vial 1.0mL |
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E0030079469 | Scientific Laboratory Supplies | PK960 | EUR 1321.26 |
Eppendorf Sterile CryoStorage Vial 1.5mL |
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Eppendorf Sterile CryoStorage Vial 2.0mL |
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E0030079485 | Scientific Laboratory Supplies | PK480 | EUR 717.06 |
Eppendorf Sterile CryoStorage Vial 4.0mL |
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E0030079493 | Scientific Laboratory Supplies | PK480 | EUR 779.76 |
Phenols Mix (2000ppm) - 1.8mL vial |
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ECS-A-006 | Scientific Laboratory Supplies | 1.8ML | EUR 60.42 |
Benzidines Mix (2000ppm) - 1.8mL vial |
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ECS-A-007 | Scientific Laboratory Supplies | 1.8ML | EUR 37.62 |
PAH Mix (2000ppm) - 1.8mL vial |
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ECS-A-032 | Scientific Laboratory Supplies | 1.8ML | EUR 118.56 |
2mL Screw Top Vial LCMS |
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CHR1046 | Scientific Laboratory Supplies | PK100 | EUR 41.3 |
Vial Clear 8mm Crimp 7x40mm |
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C4008741 | Scientific Laboratory Supplies | PK100 | EUR 28.84 |
Vial Rack 12mmx 50 Holes |
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CHR2184 | Scientific Laboratory Supplies | EACH | EUR 75.24 |
Vial Rack Acrylic 40 cavities |
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CHR2188 | Scientific Laboratory Supplies | EACH | EUR 87.78 |
2mL Crimp Top Vial+CrimpCap |
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CHR2194 | Scientific Laboratory Supplies | PK100 | EUR 45.78 |
Amber Vial Teflon Cap 4mL |
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CHR2200 | Scientific Laboratory Supplies | PK100 | EUR 89.08 |
Amber Vial Teflon Cap 40mL |
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CHR2208 | Scientific Laboratory Supplies | PK100 | EUR 180.31 |
4mL ScrewNeck Vial Amber Glass |
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CHR2222 | Scientific Laboratory Supplies | PK1000 | EUR 484.5 |
1mL Shell Vial 40x8.2 ClearGlass |
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CHR2228 | Scientific Laboratory Supplies | PK1000 | EUR 149.34 |
1mL Shell Vial 40x8.2 AmberGlass |
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CHR2230 | Scientific Laboratory Supplies | PK1000 | EUR 193.17 |
1mL Vial ClearGlass w Plug |
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2mL Vial 31.5x11.6mm Clear Glass |
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CHR2242 | Scientific Laboratory Supplies | PK1000 | EUR 165.3 |
Shell Vial 3mL 14.65x44.6mm PP |
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CHR2244 | Scientific Laboratory Supplies | PK1000 | EUR 643.32 |
40mL ScrewNeck Vial Clear Glass |
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CHR2292 | Scientific Laboratory Supplies | PK1000 | EUR 1144.56 |
Vial+Cap 40mL Clear PreAssembled |
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CHR2294 | Scientific Laboratory Supplies | PK1000 | EUR 1151.4 |
20mL Headspace Vial Clear Glass |
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CHR2452 | Scientific Laboratory Supplies | PK1000 | EUR 479.94 |
0.7mL CrimpNeck Vial Clear Glass |
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CHR2500 | Scientific Laboratory Supplies | PK1000 | EUR 208.62 |
0.7mL CrimpNeck Vial Amber Glass |
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CHR2502 | Scientific Laboratory Supplies | PK1000 | EUR 210.9 |
1.2mL CrimpNeck Vial Clear Glass |
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CHR2512 | Scientific Laboratory Supplies | PK1000 | EUR 204.06 |
2mL CrimpNeck Vial Clear GlassWO |
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1.5mL CrimpNeck Vial Clear WO |
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CHR2530 | Scientific Laboratory Supplies | PK1000 | EUR 118.56 |
Crimp Top Vial Amber FusedInsert |
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CHR2550 | Scientific Laboratory Supplies | PK1000 | EUR 1103.52 |
2.5mL CrimpNeck Vial Clear Glass |
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CHR2576 | Scientific Laboratory Supplies | PK1000 | EUR 190.38 |
20mL ScrewNeck Vial Amber Glass |
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20mL EPA ScrewNeck Vial Clear |
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30mL ScrewNeck Vial Amber Glass |
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30mL ScrewNeck Vial Clear Glass |
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40mL ScrewNeck Vial Amber Glass |
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40mL ScrewNeck Vial Clear Glass |
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60mL EPA ScrewNeck Vial Amber |
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60mL EPA ScrewNeck Vial Clear |
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8mL ScrewNeck Vial Clear Glass |
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8mL ScrewNeck Vial Amber Glass |
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12mL ScrewNeck Vial Clear Glass |
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12mL ScrewNeck Vial Amber Glass |
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16mL ScrewNeck Vial Clear Glass |
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10mL Precision Vial Clear Glass |
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CHR2646 | Scientific Laboratory Supplies | PK1000 | EUR 340.86 |
2mL ScrewNeck Vial Clear Glass |
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2mL ScrewNeck Vial Amber Glass |
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2mL ScrewNeck Vial Clear Glass |
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2mL ScrewNeck Vial Amber Glass |
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CHR2656 | Scientific Laboratory Supplies | PK1000 | EUR 166.44 |
2mL ScrewNeck Vial Clear GlassWO |
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4mL ScrewNeck Vial Amber Glass |
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4mL ScrewNeck Vial Clear Glass |
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4mL ScrewNeck Vial Amber Glass |
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2mL Snap Ring Vial Clear |
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CHR2684 | Scientific Laboratory Supplies | PK1000 | EUR 149.34 |
2mL Snap Ring Vial Amber |
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CHR2686 | Scientific Laboratory Supplies | PK1000 | EUR 151.62 |
TPX SnapRing Vial w Insert |
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CHR2704 | Scientific Laboratory Supplies | PK1000 | EUR 1013.46 |
5mL Snap Cap Vial Clear |
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CHR2720 | Scientific Laboratory Supplies | PK1000 | EUR 165.3 |
10mL Snap Cap Vial Clear |
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CHR2722 | Scientific Laboratory Supplies | PK1000 | EUR 149.57 |
15mL Snap Cap Vial Clear |
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CHR2724 | Scientific Laboratory Supplies | PK1000 | EUR 243.96 |
25mL Snap Cap Vial Clear |
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Insert SO Vial 0.1mL Conical |
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CHR2962 | Scientific Laboratory Supplies | PK1000 | EUR 232.56 |
Insert SO Vial 0.15mL Conical |
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Insert SO Vial 0.2mL Flat |
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CHR2968 | Scientific Laboratory Supplies | PK1000 | EUR 220.02 |
Insert WO Vial 0.1mL Conical |
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CHR2974 | Scientific Laboratory Supplies | PK1000 | EUR 207.97 |
Insert WO Vial spring Silanised |
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CHR2978 | Scientific Laboratory Supplies | PK1000 | EUR 1065.9 |
Insert WO Vial 0.25mL Conical |
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CHR2982 | Scientific Laboratory Supplies | PK1000 | EUR 263.34 |
Insert WO Vial 0.3mL Flat |
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CHR2984 | Scientific Laboratory Supplies | PK1000 | EUR 288.42 |
Insert WO Vial 0.3mL Silanised |
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CHR2986 | Scientific Laboratory Supplies | PK1000 | EUR 528.96 |
Trident Vial 1.75ml Tall Screwcap |
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TUB1200 | Scientific Laboratory Supplies | PK882 | EUR 276.68 |
Trident Vial 3.5ml Tall Screwcap |
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TUB1202 | Scientific Laboratory Supplies | PK666 | EUR 217.51 |
Trident Vial 7ml Tall Screwcap |
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TUB1204 | Scientific Laboratory Supplies | PK399 | EUR 191.06 |
Trident Vial 10.5ml Tall Screwcap |
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TUB1206 | Scientific Laboratory Supplies | PK399 | EUR 189.81 |
Trident Vial 14ml Tall Screwcap |
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TUB1208 | Scientific Laboratory Supplies | PK264 | EUR 130.76 |
Trident Vial 21.25ml Tall Screwcap |
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TUB1210 | Scientific Laboratory Supplies | PK231 | EUR 157.09 |
Trident Vial 28.25ml Tall Screwcap |
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TUB1212 | Scientific Laboratory Supplies | PK190 | EUR 125.74 |
Trident Vial 7ml Squat Screwcap |
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TUB1220 | Scientific Laboratory Supplies | PK264 | EUR 162.11 |
Trident Vial 14ml Squat Screwcap |
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TUB1222 | Scientific Laboratory Supplies | PK190 | EUR 130.76 |
Trident Vial 28.25ml Squat Screwcap |
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TUB1224 | Scientific Laboratory Supplies | PK140 | EUR 123.23 |
Vial Cap Seal Crimper 13mm |
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VIA1144 | Scientific Laboratory Supplies | EACH | EUR 516.42 |
Vial Cap Seal Crimper 20mm |
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VIA1146 | Scientific Laboratory Supplies | EACH | EUR 530.1 |
Cryo-Safe vial storage box |
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Z756784-4EA | Scientific Laboratory Supplies | PK4 | EUR 53.69 |
ToxOut? Endotoxin-free Glass Vial (Amber) |
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M7948-10 | Biovision | EUR 255.6 |
ToxOut? Endotoxin-free Glass Vial (Amber) |
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M7948-2 | Biovision | EUR 235.2 |
Swift Test Dpd Free Replace Vial |
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2105560 | Scientific Laboratory Supplies | PK250 | EUR 81.72 |
Low range cod vial 0-150mg |
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21258-51 | Scientific Laboratory Supplies | PK25 | EUR 103.42 |
High Range Cod Vial 0-1500Mg |
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21259-51 | Scientific Laboratory Supplies | PK25 | EUR 103.42 |
Scintillation Insert vial 4ml s/cap |
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IZ00934 | Scientific Laboratory Supplies | PK2000 | EUR 223.44 |
Nalgene 5 Vial Cryo Cane 290mm |
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CRY8322 | Scientific Laboratory Supplies | PK12 | EUR 27.36 |
Nalgene 6 Vial Cryo Cane 300mm |
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CRY8324 | Scientific Laboratory Supplies | PK48 | EUR 115.14 |
WHEATON(R) LIQUID SCINTILLATION VIAL WI |
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DWK986561-500EA | Scientific Laboratory Supplies | PK500 | EUR 304.03 |
WHEATON(R) LIQUID SCINTILLATION VIAL WI |
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DWK986754-500EA | Scientific Laboratory Supplies | PK500 | EUR 181.64 |
Semivolatile ISTD Mix (4000ppm)- 1.8mL vial |
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ECS-A-001 | Scientific Laboratory Supplies | 1.8ML | EUR 139.08 |
Semivolatile TCLP Mix (2000ppm) - 1.8mL vial |
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ECS-A-018 | Scientific Laboratory Supplies | 1.8ML | EUR 92.34 |
Semivolatile ISTD Mix (2000ppm) - 1.8mL vial |
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ECS-A-201 | Scientific Laboratory Supplies | 1.8ML | EUR 83.22 |
Kinesis Headspace Vial; 10ml Screw Top |
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CHR1005 | Scientific Laboratory Supplies | PK125 | EUR 90.77 |
Kinesis Vial Rack; 12x32mm; 50 Position |
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CHR1027 | Scientific Laboratory Supplies | EACH | EUR 27.41 |
Kinesis Crimp Vial 20mm; 100ml (95x52mm) |
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CHR1033 | Scientific Laboratory Supplies | PK88 | EUR 588.24 |
Kinesis Blood Dilution Vial; 25ml Bulk |
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CHR1062 | Scientific Laboratory Supplies | PK1000 | EUR 159.13 |
Vial Rack 24mm x 25 Holes |
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CHR2190 | Scientific Laboratory Supplies | EACH | EUR 88.92 |
Vial Rack PP for 4mL Vials |
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CHR2192 | Scientific Laboratory Supplies | EACH | EUR 26.22 |
Shell Vial 1mL Amber 8mm Plug |
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CHR2220 | Scientific Laboratory Supplies | PK200 | EUR 50.38 |
4mL Vial 44.6 x 14.6mm Clear |
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CHR2246 | Scientific Laboratory Supplies | PK1000 | EUR 212.8 |
4mL Vial 44.6 x 14.6mm Amber |
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CHR2248 | Scientific Laboratory Supplies | PK1000 | EUR 248.81 |
40mL Clear Vial Open Top Cap |
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CHR2264 | Scientific Laboratory Supplies | PK100 | EUR 197.52 |
MRQ30 Vial w PTFE/Si Cap |
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CHR2428 | Scientific Laboratory Supplies | PK100 | EUR 154.86 |
2mL SnapRing Vial Clear Glass WO |
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CHR2680 | Scientific Laboratory Supplies | PK1000 | EUR 166.44 |
SnapTop Vial 11mm 0.2mL w Insert |
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CHR2698 | Scientific Laboratory Supplies | PK1000 | EUR 522.12 |
TPX SnapRing Vial Amber w Insert |
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CHR2700 | Scientific Laboratory Supplies | PK1000 | EUR 549.48 |
Snap Top Vial 0.7mL Fused Insert |
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CHR2706 | Scientific Laboratory Supplies | PK1000 | EUR 180.12 |
SnapTop Vial 11mm w insert 300ul |
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CHR2708 | Scientific Laboratory Supplies | PK1000 | EUR 591.74 |
Thread Amber Vial 9mm 2mL Label |
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CHR2744 | Scientific Laboratory Supplies | PK1000 | EUR 176.7 |
Screw Vial 2ml w fused Inserts |
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CHR2786 | Scientific Laboratory Supplies | PK1000 | EUR 727.32 |
Shell Vial 1mL Plug Flat Bottom |
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CHR2802 | Scientific Laboratory Supplies | PK1000 | EUR 104.3 |
Shell Vial 4mL Flat Bottom Plug |
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CHR2808 | Scientific Laboratory Supplies | PK1000 | EUR 158.72 |
Snap Top Vial 11mm 2mL Clear |
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CHR2830 | Scientific Laboratory Supplies | PK1000 | EUR 201.78 |
Insert SO Vial 0.1mL w Spring |
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CHR2964 | Scientific Laboratory Supplies | PK1000 | EUR 611.78 |
Insert PP SO Vial 0.2mL Flat |
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CHR2970 | Scientific Laboratory Supplies | PK1000 | EUR 173.45 |
Metal Spring for 1mL Shell Vial |
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CHR2996 | Scientific Laboratory Supplies | PK1000 | EUR 63.84 |
Trident Vial 1.75ml Tall Plug Cap |
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TUB1250 | Scientific Laboratory Supplies | PK882 | EUR 290.36 |
The upgraded WWTP effluent couldn’t be used instantly as course of water or for agriculture functions, resulting from excessive concentrations of COD and salts within the WWTP effluent and filtrates. Nevertheless WWTP effluent or floc filtrate may very well be utilized instantly as water for the washing of sea-sand.