Orbital Biosciences online

Ultrafiltration and Microfiltration Solutions for the Biosciences

Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation.

Microfiltration adopted by focus and diafiltration on ultrafiltration membranes (Biomax-100, Biomax-10 and Ultracel-10) was used to get well extracellular laccase (EC 1.10.3.2) from tradition broth of wood-rotting fungus Cerrena unicolor. Feed, permeate, retentate and membrane wash-out options have been analysed for the presence of laccase, proteases, protein and brown impurities. A straightforward, low-cost and short-term process was proposed...